CLIA Kit for Collagen Type XII (COL12)

COL12-A1; COL12A1L; COL12A1; Collagen Alpha-1(XII)chain

  • CLIA Kit for Collagen Type XII (COL12)Packages (Simulation)
  • CLIA Kit for Collagen Type XII (COL12)Packages (Simulation)
  • CLIA Kit for Collagen Type XII (COL12)Results demonstration
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Collagen Type XII (COL12)

This assay has high sensitivity and excellent specificity for detection of Collagen Type XII (COL12).
No significant cross-reactivity or interference between Collagen Type XII (COL12) and analogues was observed.

Recovery of the CLIA Kit for Collagen Type XII (COL12)

Matrices listed below were spiked with certain level of recombinantCollagen Type XII (COL12) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type XII (COL12) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)87-9491
EDTA plasma(n=5)93-10197
heparin plasma(n=5)86-10390

Precision of the CLIA Kit for Collagen Type XII (COL12)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XII (COL12) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XII (COL12) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Collagen Type XII (COL12)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type XII (COL12) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-102%84-98%96-103%80-90%
EDTA plasma(n=5)80-95%91-104%80-91%89-97%
heparin plasma(n=5)79-90%87-94%80-102%97-104%

Stability of the CLIA Kit for Collagen Type XII (COL12)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided of the CLIA Kit for Collagen Type XII (COL12)

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
Substrate A1×10mLSubstrate B1×2mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary of the CLIA Kit for Collagen Type XII (COL12)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Collagen Type XII (COL12)

Test principle of the CLIA Kit for Collagen Type XII (COL12)

The microplate provided in this kit has been pre-coated with an antibody specific to Collagen Type XII (COL12). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Collagen Type XII (COL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Collagen Type XII (COL12) level in the sample or standard.;

Related products

Catalog No.Organism species: Homo sapiens (Human)Applications (RESEARCH USE ONLY!)
RPC158Hu01Recombinant Collagen Type XII (COL12)Positive Control; Immunogen; SDS-PAGE; WB.
PAC158Hu01Polyclonal Antibody to Collagen Type XII (COL12)WB; IHC; ICC; IP.
MAC158Hu22Monoclonal Antibody to Collagen Type XII (COL12)WB; IHC; ICC; IP.
SEC158HuELISA Kit for Collagen Type XII (COL12)Enzyme-linked immunosorbent assay for Antigen Detection.
SCC158HuCLIA Kit for Collagen Type XII (COL12)Chemiluminescent immunoassay for Antigen Detection.
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