CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

CCL3-L1; LD78; SCYA3L; SCYA3L1; MIP1AP; MIP1-Alpha-P; Small Inducible Cytokine A3-Like 1; Macrophage Inflammatory Protein 1-Alpha-P; Tonsillar Lymphocyte LD78 Beta

  • CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Packages (Simulation)
  • CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Packages (Simulation)
  • CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1).
No significant cross-reactivity or interference between Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and analogues was observed.

Recovery of the CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

Matrices listed below were spiked with certain level of recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-105 96
EDTA plasma(n=5) 80-88 84
heparin plasma(n=5) 90-104 95

Precision of the CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-93% 80-98% 82-104% 90-98%
EDTA plasma(n=5) 95-102% 80-101% 90-102% 90-103%
heparin plasma(n=5) 91-104% 82-97% 97-104% 85-94%

Stability of the CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPA026Mu02 Recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA026Mu01 Recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA026Mu02 Polyclonal Antibody to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) WB; IHC; ICC; IP.
PAA026Mu01 Polyclonal Antibody to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) WB; IHC; ICC; IP.
SEA026Mu ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA026Mu CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Chemiluminescent immunoassay for Antigen Detection.
LMA026Mu Multiplex Assay Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK