Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)
Speckled 100 kDa; Nuclear dot-associated Sp100 protein
- Product No.PAA326Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA326Hu01-Recombinant Sp100 Nuclear Antigen (Sp100)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species Moren/a
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 220
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For more details, please contact local distributors! US$ 3140
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Figure: FITC staining on IHC-P;
Sample: MCF7 cells.
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Figure. Western Blot; Sample: Recombinant Sp100, Human.
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate
Primary Ab: 5ug/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: Sp100 knockout Hela cell lysate;
Predicted MW: 50,51,53,54,78,100,102kd
Observed MW: 50kd
Primary Ab: 5µg/ml Rabbit Anti-Human Sp100 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)
The antibody is a rabbit polyclonal antibody raised against Sp100. It has been selected for its ability to recognize Sp100 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)
Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA326Hu01 | Recombinant Sp100 Nuclear Antigen (Sp100) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA326Hu01 | Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100) | WB,IHC |