Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)

PKU; PKU1; Phe-4-monooxygenase

  • Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)Packages (Simulation)
  • Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)Packages (Simulation)
  • Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)Figure. DAB staining on IHC-P; Samples: Human Kidney Tissue.
  • Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PAH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC750Hu01.jpgFigure. Western Blot; Sample: Recombinant PAH, Human.
  • Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)Figure. Western Blot; Lane1: Human Liver Tissue; Lane2: Porcine Kidney Tissue; Lane3: Mouse Gallbladder Tissue; Lane4: Human Hela Cells.
  • Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: PAH knockout Hela cell lysate;
    Predicted MW: 51kd
    Observed MW: 55kd
    Primary Ab: 2µg/ml Rabbit Anti-Human PAH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)

The antibody is a rabbit polyclonal antibody raised against PAH. It has been selected for its ability to recognize PAH in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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