Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase

  • Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)Packages (Simulation)
  • Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)Packages (Simulation)
  • PAC027Hu01.jpgWB Image
  • Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)Figure. Western Blot; Sample: Human Jurkat Cells.
  • Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)Knockout Varification:
    Lane 1: Wild-type Jurkat cell lysate;
    Lane 2: MGMT knockout Jurkat cell lysate;
    Predicted MW: 21kd
    Observed MW: 22kd
    Primary Ab: 3µg/ml Rabbit Anti-Human MGMT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

The antibody is a rabbit polyclonal antibody raised against MGMT. It has been selected for its ability to recognize MGMT in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No.Organism species: Homo sapiens (Human)Applications (RESEARCH USE ONLY!)
RPC027Hu01Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT)Positive Control; Immunogen; SDS-PAGE; WB.
PAC027Hu01Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)WB; IHC; ICC; IP.
MAC027Hu22Monoclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)WB; IHC; ICC; IP.
SEC027HuELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT)Enzyme-linked immunosorbent assay for Antigen Detection.
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