Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)
CGI-25; eNOS-interacting protein
- Product No.PAA330Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA330Hu01-Recombinant Nitric Oxide Synthase Interacting Protein (NOSIP)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 94
For more details, please contact local distributors!US$ 220
For more details, please contact local distributors! US$ 314
For more details, please contact local distributors! US$ 785
For more details, please contact local distributors! US$ 3140
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NOSIP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NOSIP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NOSIP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant NOSIP, Human.
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Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: NOSIP knockout 293T cell lysate;
Predicted MW: 33kd
Observed MW: 35kd
Primary Ab: 5µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human A431 cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Liver lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human NOSIP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)
The antibody is a rabbit polyclonal antibody raised against NOSIP. It has been selected for its ability to recognize NOSIP in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)
Western blotting: 0.5-5µg/mL;
Immunohistochemistry: 5-50µg/mL;
Immunocytochemistry: 5-50µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA330Hu01 | Recombinant Nitric Oxide Synthase Interacting Protein (NOSIP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA330Hu01 | Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) | WB,IHC |
| MAA330Hu21 | Monoclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) | WB; IHC; ICC; IP. |
| SEA330Hu | ELISA Kit for Nitric Oxide Synthase Interacting Protein (NOSIP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA330Hu | Multiplex Assay Kit for Nitric Oxide Synthase Interacting Protein (NOSIP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |