Polyclonal Antibody to SMAD family member 3 (SMAD3)
JV15-2; MADH3; Mothers Against Decapentaplegic Homolog 3
- Product No.PAC123Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC123Hu01-Recombinant SMAD family member 3 (SMAD3)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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IHC Images
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Figure. Western Blot; Sample: Recombinant Smad3, Human.
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Western Blot; Sample: Rat Pancreas lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type PC3 cell lysate;
Lane 2: Smad3 knockout PC3 cell lysate;
Predicted MW: 26,36,43,48kd
Observed MW: 43kd
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human PC3 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to SMAD family member 3 (SMAD3)
The antibody is a rabbit polyclonal antibody raised against SMAD3. It has been selected for its ability to recognize SMAD3 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to SMAD family member 3 (SMAD3)
Western blottin:g: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to SMAD family member 3 (SMAD3)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to SMAD family member 3 (SMAD3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPC123Hu01 | Recombinant SMAD family member 3 (SMAD3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC123Hu01 | Polyclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu21 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu22 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu23 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |