Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)

HLA-DRA; HLADRa; HLA Class II Histocompatibility Antigen,DR Alpha Chain

  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Packages (Simulation)
  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Packages (Simulation)
  • PAC166Hu01.jpgWB Image
  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Knockout Varification:
    Lane 1: Wild-type Jurkat cell lysate;
    Lane 2: MHCDRa knockout Jurkat cell lysate;
    Predicted MW: 29kDa
    Observed MW: 25kDa;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Western Blot; Sample: Human Raji cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Western Blot; Sample: Human Jurkat cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Western Blot; Sample: Mouse Spleen lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Western Blot; Sample: Mouse Kidney lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Western Blot; Sample: Mouse Liver lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human HLA-DR Polyclonal Antibody (Catalog # PAC166Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)

The antibody is a rabbit polyclonal antibody raised against MHCDRa. It has been selected for its ability to recognize MHCDRa in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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