Polyclonal Antibody to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)
HSP-A1L; HSPA1-L; HSP70-HOM; Hum70t
- Product No.PAB062Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB062Hu02-Recombinant Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.59mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors! US$ 3320
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Packages (Simulation)
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Packages (Simulation)
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IHC Images
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Figure. Western Blot; Sample: Recombinant HSPA1L, Human.
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Western Blot; Sample: Raji cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HSPA1L Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Knockout Varification:
Lane 1: Wild-type Raji cell lysate;
Lane 2: HSPA1L knockout Raji cell lysate;
Predicted MW: 70kd
Observed MW: 70kd
Primary Ab: 5µg/ml Rabbit Anti-Human HSPA1L Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Hela cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HSPA1L Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: HepG2 cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HSPA1L Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human HSPA1L Polyclonal Antibody (Catalog # PAB062Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15).Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)
The antibody is a rabbit polyclonal antibody raised against HSPA1L. It has been selected for its ability to recognize HSPA1L in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)
Western blotting: 0.5-2µg/mL;1:300-1200
Immunohistochemistry: 5-20µg/mL;1:30-120
Immunocytochemistry: 5-20µg/mL;1:30-120
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service