Polyclonal Antibody to Enolase, Neuron Specific (NSE)

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Packages (Simulation)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Packages (Simulation)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)DAB staining on IHC-P; Samples: Rat Pancreas Tissue
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)DAB staining on IHC-P;
    Samples: Rat Cerebrum Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat NSE Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)DAB staining on IHC-P;
    Samples: Rat Testis Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat NSE Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA537Ra01.jpgFigure. Western Blot; Sample: Recombinant NSE, Rat.
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Figure. Western Blot; Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Brain Tissue; Lane3: Human HepG2 Cells; Lane4: Human Hela Cells.
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: NSE knockout HepG2 cell lysate;
    Predicted MW: 47kd
    Observed MW: 52kd
    Primary Ab: 1µg/ml Rabbit Anti-Rat NSE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-rat NSE Polyclonal Antibody (Catalog # PAA537Ra01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

The antibody is a rabbit polyclonal antibody raised against NSE. It has been selected for its ability to recognize NSE in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

Western blotting: 0.5-2µg/mL;1:190-760
Immunohistochemistry: 5-20µg/mL;1:19-76
Immunocytochemistry: 5-20µg/mL;1:19-76
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No.Organism species: Rattus norvegicus (Rat)Applications (RESEARCH USE ONLY!)
RPA537Ra01Recombinant Enolase, Neuron Specific (NSE)Positive Control; Immunogen; SDS-PAGE; WB.
PAA537Ra01Polyclonal Antibody to Enolase, Neuron Specific (NSE)WB; IHC; ICC; IP.
LAA537Ra81FITC-Linked Polyclonal Antibody to Enolase, Neuron Specific (NSE)WB; IHC; ICC; IF.
LAA537Ra71Biotin-Linked Polyclonal Antibody to Enolase, Neuron Specific (NSE)WB; IHC; ICC.
MAA537Ra21Monoclonal Antibody to Enolase, Neuron Specific (NSE)WB; IHC; ICC; IP.
SEA537RaELISA Kit for Enolase, Neuron Specific (NSE)Enzyme-linked immunosorbent assay for Antigen Detection.
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