Polyclonal Antibody to Enolase, Non Neuronal (NNE)

ENO1; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) Packages (Simulation)
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) Packages (Simulation)
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse NNE Anti
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) DAB staining on IHC-P;
    Samples: Mouse Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB449Mu01.jpg WB Image
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: NNE knockout MCF7 cell lysate;
    Predicted MW: 36,47kDa
    Observed MW: 50kDa
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) Western Blot; Sample: Mouse Placenta lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) Western Blot; Sample: Rat Mammary gland lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) Western Blot; Sample: Mouse Colon lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Non Neuronal (NNE) Western Blot; Sample: Mouse Kidney lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Enolase, Non Neuronal (NNE)

The antibody is a rabbit polyclonal antibody raised against NNE. It has been selected for its ability to recognize NNE in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Enolase, Non Neuronal (NNE)

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Enolase, Non Neuronal (NNE)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Enolase, Non Neuronal (NNE)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Western Blot (WB) Experiment Service
Real Time PCR Experimental Service

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