Polyclonal Antibody to Enolase, Neuron Specific (NSE)

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Packages (Simulation)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Packages (Simulation)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Used in DAB staining on fromalin fixed paraffin-embedded Brain tissue
  • PAA537Mu02.jpgWB Image
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: NSE knockout HepG2 cell lysate;
    Predicted MW: 47kDa
    Observed MW: 50kDa
    Primary Ab: 2µg/ml Rabbit Anti-Mouse NSE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Western Blot; Sample: Human 293T cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NSE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE)Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NSE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

The antibody is a rabbit polyclonal antibody raised against NSE. It has been selected for its ability to recognize NSE in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.Organism species: Mus musculus (Mouse)Applications (RESEARCH USE ONLY!)
RPA537Mu02Recombinant Enolase, Neuron Specific (NSE)Positive Control; Immunogen; SDS-PAGE; WB.
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PGA537Mu01Primer Pair for Enolase, Neuron Specific (NSE)PCR
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