Polyclonal Antibody to Cystatin B (CSTB)

Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor

  • Polyclonal Antibody to Cystatin B (CSTB) Packages (Simulation)
  • Polyclonal Antibody to Cystatin B (CSTB) Packages (Simulation)
  • Polyclonal Antibody to Cystatin B (CSTB) DAB staining on IHC-P; Samples: Human Thyroid Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CSTB Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cystatin B (CSTB) DAB staining on IHC-P;
    Sample: Human Esophagus cancer Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human CSTB Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cystatin B (CSTB) FITC staining on IF;
    Sample: Human MCF7 cell;
    Primary Ab: 10µg/ml Rabbit Anti-Human CSTB Antibody
    Second Ab: 1.3µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAJ322Hu01.jpg Figure. Western Blot; Sample: Recombinant CSTB, Human.
  • Polyclonal Antibody to Cystatin B (CSTB) Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: CSTB knockout HepG2 cell lysate;
    Predicted MW: 11kDa
    Observed MW: 14kDa
    Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cystatin B (CSTB) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cystatin B (CSTB) Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cystatin B (CSTB) Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: MCF7 cell lysate; Lane3: A549 cell lysate;
    Primary Ab: 0.05µg/ml Rabbit Anti-Human CSTB Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cystatin B (CSTB) Human A431 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CSTB Polyclonal Antibody (Catalog PAJ322Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Cystatin B (CSTB)

The antibody is a rabbit polyclonal antibody raised against CSTB. It has been selected for its ability to recognize CSTB in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Cystatin B (CSTB)

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
For flow cytometry: 0.5-2 µg per 10^6 cells in 100 µl volume.
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Cystatin B (CSTB)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Cystatin B (CSTB)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPJ322Hu01 Recombinant Cystatin B (CSTB) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ322Hu01 Polyclonal Antibody to Cystatin B (CSTB) WB;IHC;ICC/IF;FCM
MAJ322Hu21 Monoclonal Antibody to Cystatin B (CSTB) WB; IHC; ICC; IP.
SEJ322Hu ELISA Kit for Cystatin B (CSTB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ322Hu Multiplex Assay Kit for Cystatin B (CSTB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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