Polyclonal Antibody to Caspase 8 (CASP8)

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

  • Polyclonal Antibody to Caspase 8 (CASP8)Packages (Simulation)
  • Polyclonal Antibody to Caspase 8 (CASP8)Packages (Simulation)
  • Polyclonal Antibody to Caspase 8 (CASP8)Used in DAB staining on fromalin fixed paraffin-embedded Liver tissue
  • Polyclonal Antibody to Caspase 8 (CASP8)Figure:FITC staining on IHC-P
    Simple: Hela cells
  • PAA853Hu01.jpgFigure. Western Blot; Sample: Recombinant CASP8, Human.
  • Polyclonal Antibody to Caspase 8 (CASP8)Figure. Western Blot; Sample: Human HepG2 Cells.
  • Polyclonal Antibody to Caspase 8 (CASP8)Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: CASP8 knockout Hela cell lysate;
    Predicted MW: 26-32,46,54-62kd
    Observed MW: 35kd
    Primary Ab: 1µg/ml Rabbit Anti-Human CASP8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 8 (CASP8)HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human CASP8 Polyclonal Antibody (Catalog # PAA853Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Caspase 8 (CASP8)

The antibody is a rabbit polyclonal antibody raised against CASP8. It has been selected for its ability to recognize CASP8 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Caspase 8 (CASP8)

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Caspase 8 (CASP8)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Caspase 8 (CASP8)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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