Active S100 Calcium Binding Protein A6 (S100A6)
S100-A6; 2A9; 5B10; CABP; CACY; PRA; MLN 4; Calcyclin; Growth factor-inducible protein 2A9; Prolactin receptor-associated protein
- Product No.APB769Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point5.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 320
For more details, please contact local distributors!US$ 800
For more details, please contact local distributors! US$ 1600
For more details, please contact local distributors! US$ 4800
For more details, please contact local distributors! US$ 12000
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure . Gene Sequencing (extract)
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SDS-PAGE
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Figure. Western Blot; Sample: Recombinant S100A6, Human.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active S100 Calcium Binding Protein A6 (S100A6)
S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE (Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active S100 Calcium Binding Protein A6 (S100A6)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active S100 Calcium Binding Protein A6 (S100A6)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active S100 Calcium Binding Protein A6 (S100A6)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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