Active Protein Kinase R (PKR)
PRKR; PRK-R; EIF2AK2; EIF2AK1; Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; P1/eIF-2A kinase
- Product No.APA520Hu01
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 80%
- Isoelectric Point8.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 376
For more details, please contact local distributors!US$ 940
For more details, please contact local distributors!US$ 1880
For more details, please contact local distributors!US$ 5640
For more details, please contact local distributors!US$ 14100
For more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant PKR, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Protein Kinase R (PKR)
Protein Kinase R (PKR) is activated by double-stranded RNA (dsRNA), the synthesis of which is caused virally. PKR can also be activated by the protein PACT or by heparin. It plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Besides, Cyclin Dependent Kinase 1 (CDK1) has been identified as an interactor of PKR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PKR and recombinant human CDK1. Briefly, PKR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PKR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of PKR and CDK1 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Protein Kinase R (PKR)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Protein Kinase R (PKR)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Protein Kinase R (PKR)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|APA520Hu01||Active Protein Kinase R (PKR)||Cell culture; Activity Assays.|
|RPA520Hu01||Recombinant Protein Kinase R (PKR)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA520Hu01||Polyclonal Antibody to Protein Kinase R (PKR)||WB; IHC; ICC; IP.|
|MAA520Hu22||Monoclonal Antibody to Protein Kinase R (PKR)||WB; IHC; ICC; IP.|
|SEA520Hu||ELISA Kit for Protein Kinase R (PKR)||Enzyme-linked immunosorbent assay for Antigen Detection.|