Active Protein Kinase R (PKR)
PRKR; PRK-R; EIF2AK2; EIF2AK1; Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; P1/eIF-2A kinase
- Product No.APA520Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 80%
- Isoelectric Point8.9
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant PKR, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Protein Kinase R (PKR)
Protein Kinase R (PKR) is activated by double-stranded RNA (dsRNA), the synthesis of which is caused virally. PKR can also be activated by the protein PACT or by heparin. It plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Besides, Cyclin Dependent Kinase 1 (CDK1) has been identified as an interactor of PKR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PKR and recombinant human CDK1. Briefly, PKR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PKR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of PKR and CDK1 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Protein Kinase R (PKR)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Protein Kinase R (PKR)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Protein Kinase R (PKR)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Molecular Mass Marker for Protein
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
Real Time PCR Experimental Service
BCA Protein Quantification Kit
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|APA520Hu01||Active Protein Kinase R (PKR)||Cell culture; Activity Assays.|
|RPA520Hu01||Recombinant Protein Kinase R (PKR)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA520Hu01||Polyclonal Antibody to Protein Kinase R (PKR)||WB; IHC; ICC; IP.|
|MAA520Hu22||Monoclonal Antibody to Protein Kinase R (PKR)||WB; IHC; ICC; IP.|
|SEA520Hu||ELISA Kit for Protein Kinase R (PKR)||Enzyme-linked immunosorbent assay for Antigen Detection.|