Active Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
- Product No.APA899Ra61
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point4.3
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 352
For more details, please contact local distributors!US$ 880
For more details, please contact local distributors! US$ 1760
For more details, please contact local distributors! US$ 5280
For more details, please contact local distributors! US$ 13200
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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WB Image
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Osteopontin (OPN)
Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappaB pathways in MCF-7 breast cancer cell line. To test the bioactivity of recombinant rat OPN, MCF-7 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of recombinant rat OPN for 48h and MCP-1 levels in the cell supernatant were determined by ELISA (SEA087Hu). MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN which was shown in Figure1, the EC50 was 9.83 ug/ml.
USAGE of the Active Osteopontin (OPN)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Osteopontin (OPN)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Osteopontin (OPN)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
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Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
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Buffer
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Spike RBD Protein (S-RBD)
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Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPA899Ra01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA899Ra61 | Eukaryotic Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA899Ra61 | Active Osteopontin (OPN) | Cell culture; Activity Assays. |
| PAA899Ra01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAA899Ra06 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| MAA899Ra21 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| SEA899Ra | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA899Ra | Multiplex Assay Kit for Osteopontin (OPN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA899Ra01 | ELISA Kit DIY Materials for Osteopontin (OPN) | Main materials for "Do It (ELISA Kit) Yourself". |