Active Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
- Product No.APA899Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point4.3
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 94
For more details, please contact local distributors!US$ 236
For more details, please contact local distributors! US$ 472
For more details, please contact local distributors! US$ 1416
For more details, please contact local distributors! US$ 3540
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Gene sequencing
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SDS-PAGE
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Figure. Western Blot; Sample: Recombinant OPN, Human.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Osteopontin (OPN)
Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which would promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without OPN(100ng/ml、200ng/ml) for 24h and MCP-1 levels in the cell supernatant were determined by ELISA.
MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1.
USAGE of the Active Osteopontin (OPN)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Osteopontin (OPN)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Osteopontin (OPN)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| EPA899Hu61 | Eukaryotic Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA899Hu02 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA899Hu62 | Eukaryotic Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA899Hu61 | Active Osteopontin (OPN) | Cell culture; Activity Assays. |
| PAA899Hu06 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAA899Hu02 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| LAA899Hu71 | Biotin-Linked Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC. |
| MAA899Hu22 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| SEA899Hu | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA899Hu | ELISA Kit for Anti-Osteopontin Antibody (Anti-OPN) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA899Hu | CLIA Kit for Osteopontin (OPN) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA899Hu | Multiplex Assay Kit for Osteopontin (OPN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA899Hu01 | ELISA Kit DIY Materials for Osteopontin (OPN) | Main materials for "Do It (ELISA Kit) Yourself". |