Active Chondroitin Sulfate Proteoglycan 4 (CSPG4)

CSPG4; MSK16; HMW-MAA; MCSP; MCSPG; MEL-CSPG; NG2; Chondroitin Sulfate Proteoglycan 4; Melanoma chondroitin sulfate proteoglycan

  • Active Chondroitin Sulfate Proteoglycan 4 (CSPG4) Packages (Simulation)
  • Active Chondroitin Sulfate Proteoglycan 4 (CSPG4) Packages (Simulation)
  • APB134Hu02.png Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Chondroitin Sulfate Proteoglycan 4 (CSPG4)

Figure. The binding activity of MCSP with GAL8.
Melanoma-associated chondroitin sulfate proteoglycan (MCSP) is a chondroitin sulfate proteoglycan in humans. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. Besides, Galectin-8 (GAL8) has been identified as an interactor of MCSP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MCSP and recombinant human GAL8. Briefly, MCSP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GAL8-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MCSP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MCSP and GAL8 was shown in Figure 1, and this effect was in a dose dependent manner.

Figure. Western Blot

USAGE of the Active Chondroitin Sulfate Proteoglycan 4 (CSPG4)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Chondroitin Sulfate Proteoglycan 4 (CSPG4)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Chondroitin Sulfate Proteoglycan 4 (CSPG4)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPB134Hu02 Recombinant Chondroitin Sulfate Proteoglycan 4 (CSPG4) Positive Control; Immunogen; SDS-PAGE; WB.
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