Active Macrophage Migration Inhibitory Factor (MIF)

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

  • Active Macrophage Migration Inhibitory Factor (MIF)Packages (Simulation)
  • Active Macrophage Migration Inhibitory Factor (MIF)Packages (Simulation)
  • APA698Mu01.pngFigure. SDS-PAGE
  • Active Macrophage Migration Inhibitory Factor (MIF)Figure. Western Blot
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Macrophage Migration Inhibitory Factor (MIF)

Figure. The binding activity of MIF with MHCDG.
Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MIF and recombinant mouse MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Macrophage Migration Inhibitory Factor (MIF)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Macrophage Migration Inhibitory Factor (MIF)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Macrophage Migration Inhibitory Factor (MIF)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No.Organism species: Mus musculus (Mouse)Applications (RESEARCH USE ONLY!)
APA698Mu01Active Macrophage Migration Inhibitory Factor (MIF)Cell culture; Activity Assays.
RPA698Mu02Recombinant Macrophage Migration Inhibitory Factor (MIF)Positive Control; Immunogen; SDS-PAGE; WB.
RPA698Mu01Recombinant Macrophage Migration Inhibitory Factor (MIF)Positive Control; Immunogen; SDS-PAGE; WB.
PAA698Mu01Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)WB; IHC; ICC; IP.
PAA698Mu02Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)WB; IHC; ICC; IP.
SEA698MuELISA Kit for Macrophage Migration Inhibitory Factor (MIF)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA698MuCLIA Kit for Macrophage Migration Inhibitory Factor (MIF)Chemiluminescent immunoassay for Antigen Detection.
GUESTBOOK