Active Interleukin 29 (IL29)
FNL1; Interferon,Lambda 1; Cytokine Zcyto21
- Product No.APC029Hu01
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point9.3
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 331
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- Packages (Simulation)
- Packages (Simulation)
- Figure. SDS-PAGE
- Figure. Western Blot
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 29 (IL29)
Figure. The binding activity of IL29 with ATP2B2.
Interleukin-29 (IL-29) is a member of the helical cytokine family and is a type III interferon. It is also known as IFNλ1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. Besides, ATPase, Ca Transporting, Plasma Membrane 2 (ATP2B2) has been identified as an interactor of IL29, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL29 and recombinant human ATP2B2. Briefly, IL29 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to ATP2B2-coated microtiter wells and incubated for 2h at 37 ℃. Wells were washed with PBST and incubated for 1h with anti-IL29 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL29 and ATP2B2 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Interleukin 29 (IL29)
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 29 (IL29)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 29 (IL29)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Molecular Mass Marker for Protein
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
Real Time PCR Experimental Service
|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|RPC029Hu01||Recombinant Interleukin 29 (IL29)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC029Hu01||Active Interleukin 29 (IL29)||Cell culture; Activity Assays.|
|PAC029Hu01||Polyclonal Antibody to Interleukin 29 (IL29)||WB; IHC; ICC; IP.|
|MAC029Hu22||Monoclonal Antibody to Interleukin 29 (IL29)||WB; IHC; ICC; IP.|
|SEC029Hu||ELISA Kit for Interleukin 29 (IL29)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC029Hu||CLIA Kit for Interleukin 29 (IL29)||Chemiluminescent immunoassay for Antigen Detection.|