Active Interleukin 2 Receptor Beta (IL2Rb)

CD122; IL2R-b; IL2-RB; P70-75; High affinity IL-2 receptor subunit beta; Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta

  • Active Interleukin 2 Receptor Beta (IL2Rb) Packages (Simulation)
  • Active Interleukin 2 Receptor Beta (IL2Rb) Packages (Simulation)
  • APA229Mu01.jpg SDS-PAGE
  • Active Interleukin 2 Receptor Beta (IL2Rb) Figure. Western Blot; Sample: Recombinant IL2Rb, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Interleukin 2 Receptor Beta (IL2Rb)

IL2RB (interleukin-2 receptor subunit beta) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, which binds and responds to a cytokine called IL-2. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant mouse IL2RB with recombinant mouse IL2. Briefly, IL2RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL2RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL2RB with IL2 was shown in Figure 1 and this effect was in a dose dependent manner.

USAGE of the Active Interleukin 2 Receptor Beta (IL2Rb)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Interleukin 2 Receptor Beta (IL2Rb)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Interleukin 2 Receptor Beta (IL2Rb)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
APA229Mu01 Active Interleukin 2 Receptor Beta (IL2Rb) Cell culture; Activity Assays.
RPA229Mu01 Recombinant Interleukin 2 Receptor Beta (IL2Rb) Positive Control; Immunogen; SDS-PAGE; WB.
PAA229Mu01 Polyclonal Antibody to Interleukin 2 Receptor Beta (IL2Rb) WB; IHC; ICC; IP.
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