Active Interferon Gamma (IFNg)

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
  • Active Interferon Gamma (IFNg)Packages (Simulation)
  • Active Interferon Gamma (IFNg)Packages (Simulation)
  • Active Interferon Gamma (IFNg)Gene sequencing
  • APA049Mu01.jpgSDS-PAGE
  • Active Interferon Gamma (IFNg)Western Blot, Sample: APA049Mu01; Antibody: PAA049Mu01
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST

IFN-γ is an important activator of macrophages, it promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 246.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 246.7 cells were incubated in DMEM with IFN-γ (2ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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