Active Integrin Associated Protein (IAP)

CD47; MER6; OA3; Antigen Identified By Monoclonal Antibody 1D8; Antigenic Surface Determinant Protein OA3; Rh-Related Antigen

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
  • Active Integrin Associated Protein (IAP)Packages (Simulation)
  • Active Integrin Associated Protein (IAP)Packages (Simulation)
  • APB014Mu01.jpgSDS-PAGE
  • Active Integrin Associated Protein (IAP)Figure. Western Blot; Sample: Recombinant IAP, Mouse.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST

Integrin Associated Protein (IAP) also known as Cluster of Differentiation 47 (CD47) is a transmembrane protein. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells. Besides, Signal Regulatory Protein Alpha (SIRPa) has been identified as an interactor of IAP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IAP and recombinant mouse SIRPa. Briefly, IAP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIRPa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IAP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IAP and SIRPa was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.Organism species: Mus musculus (Mouse)Applications
APB014Mu01Active Integrin Associated Protein (IAP)Cell culture; Activity Assays.
RPB014Mu01Recombinant Integrin Associated Protein (IAP)Positive Control; Immunogen; SDS-PAGE; WB.
PAB014Mu01Polyclonal Antibody to Integrin Associated Protein (IAP)WB; IHC; ICC; IP.
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