Active Integrin Associated Protein (IAP)
CD47; MER6; OA3; Antigen Identified By Monoclonal Antibody 1D8; Antigenic Surface Determinant Protein OA3; Rh-Related Antigen
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
- Product No.APB014Mu01
- Organism SpeciesMus musculus (Mouse)Same name, Different species.
- Brand OwnerCLOUD-CLONE CORP.(CCC, USA)
- ManufacturerCLOUD-CLONE CORP.(CCC, WUH)
- Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point7.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 280
For negotiated price and more details, please contact local distributors!US$ 700
For negotiated price and more details, please contact local distributors!US$ 1400
For negotiated price and more details, please contact local distributors!US$ 4200
For negotiated price and more details, please contact local distributors!US$ 105000
For negotiated price and more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant IAP, Mouse.
- ISO9001: 2008, ISO13485: 2003 Registered
Integrin Associated Protein (IAP) also known as Cluster of Differentiation 47 (CD47) is a transmembrane protein. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells. Besides, Signal Regulatory Protein Alpha (SIRPa) has been identified as an interactor of IAP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IAP and recombinant mouse SIRPa. Briefly, IAP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIRPa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IAP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IAP and SIRPa was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Catalog No.||Organism species: Mus musculus (Mouse)||Applications|
|APB014Mu01||Active Integrin Associated Protein (IAP)||Cell culture; Activity Assays.|
|RPB014Mu01||Recombinant Integrin Associated Protein (IAP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB014Mu01||Polyclonal Antibody to Integrin Associated Protein (IAP)||WB; IHC; ICC; IP.|