Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

  • Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)Packages (Simulation)
  • Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)Packages (Simulation)
  • APA054Hu01.jpgFigure. SDS-PAGE
  • Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)Figure. Western Blot
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

Figure. The binding activity of IGFBP3 with EGFR.
Insulin-like growth factor-binding protein 3, also known as IGFBP3 is one of six IGF binding proteins (IGFBP1 to IGFBP6) that have highly conserved structures and bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. Within tissues, IGFBP3 can bind IGF1 and IGF2 released by many cell types, and block their access to the IGF-1 receptor (IGF1R), which is activated by both IGFs. IGFBP3 also interacts with cell-surface proteins, affecting cell signaling from outside the cell or after internalization, and also enters the cell nucleus where it binds to nuclear hormone receptors and other ligands. Besides, Epidermal Growth Factor Receptor (EGFR) has been identified as an interactor of IGFBP3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IGFBP3 and recombinant human EGFR. Briefly, IGFBP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EGFR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IGFBP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IGFBP3 and EGFR was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No.Organism species: Homo sapiens (Human)Applications (RESEARCH USE ONLY!)
APA054Hu01Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)Cell culture; Activity Assays.
RPA054Hu01Recombinant Insulin Like Growth Factor Binding Protein 3 (IGFBP3)Positive Control; Immunogen; SDS-PAGE; WB.
PAA054Hu01Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 3 (IGFBP3)WB; IHC; ICC; IP.
LAA054Hu71Biotin-Linked Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 3 (IGFBP3)WB; IHC; ICC.
MAA054Hu22Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 3 (IGFBP3)WB; IHC; ICC; IP.
SEA054HuELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)Enzyme-linked immunosorbent assay for Antigen Detection.
KSA054Hu01ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK