Active Granzyme M (GZMM)

GZM-M; LMET1; MET1; Hu-Met-1; Met-ase; Lymphocyte Met Ase 1; Met-1 serine protease; Natural killer cell granular protease

  • Active Granzyme M (GZMM) Packages (Simulation)
  • Active Granzyme M (GZMM) Packages (Simulation)
  • Active Granzyme M (GZMM) Gene sequencing
  • APA431Hu01.jpg Figure. SDS-PAGE
  • Active Granzyme M (GZMM) Figure. Western Blot; Sample: Recombinant GZMM, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Granzyme M (GZMM)

GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets a-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Human granzyme M is synthesized as a precursor (264 residues) with a signal peptide (residues 123), a propeptide (residues 24-25) and a mature chain (residues 26-257). The purified recombinant human Granzyme M consists of residues 26 to 257 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMM (diluted by assay buffer) to 50 µL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMM, replace with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme M is >80 pmol/min/µg.

USAGE of the Active Granzyme M (GZMM)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Granzyme M (GZMM)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Granzyme M (GZMM)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
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