Active Glutathione S Transferase Mu 1 (GSTm1)
GST-M1; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; MU1; GST HB subunit 4
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
- Product No.APA658Ra01
- Organism SpeciesRattus norvegicus (Rat)Same name, Different species.
- Brand OwnerCLOUD-CLONE CORP.(CCC, USA)
- ManufacturerCLOUD-CLONE CORP.(CCC, WUH)
- Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point8.3
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 376
For negotiated price and more details, please contact local distributors!US$ 940
For negotiated price and more details, please contact local distributors!US$ 1880
For negotiated price and more details, please contact local distributors!US$ 5640
For negotiated price and more details, please contact local distributors!US$ 141000
For negotiated price and more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant GSTm1, Rat.
- ISO9001: 2008, ISO13485: 2003 Registered
Glutathione S-transferase Mu 1 (GSTM1) belongs to the mu class. GSTM1 can detoxifying carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Besides, uncoupling Protein 1 (UCP1) has been identified as an interactor of GSTM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GSTM1 and recombinant rat UCP1. Briefly, GSTM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSTM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTM1 and UCP1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Catalog No.||Organism species: Rattus norvegicus (Rat)||Applications|
|RPA658Ra01||Recombinant Glutathione S Transferase Mu 1 (GSTm1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA658Ra01||Active Glutathione S Transferase Mu 1 (GSTm1)||Cell culture; Activity Assays.|
|PAA658Ra01||Polyclonal Antibody to Glutathione S Transferase Mu 1 (GSTm1)||WB; IHC; ICC; IP.|
|LAA658Ra71||Biotin-Linked Polyclonal Antibody to Glutathione S Transferase Mu 1 (GSTm1)||WB; IHC; ICC.|
|SEA658Ra||ELISA Kit for Glutathione S Transferase Mu 1 (GSTm1)||Enzyme-linked immunosorbent assay for Antigen Detection.|