Active Fibroblast Growth Factor 22 (FGF22)
- Product No.APC909Mu01
- Organism SpeciesMus musculus (Mouse)Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point11.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 280
For more details, please contact local distributors!US$ 700
For more details, please contact local distributors!US$ 1400
For more details, please contact local distributors!US$ 4200
For more details, please contact local distributors!US$ 10500
For more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant FGF22, Mouse.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Fibroblast Growth Factor 22 (FGF22)
FGF22 (Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF22 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF22. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of 3T3 cells after incubation with FGF22 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF22 for 48h. The result was shown in Figure 2. It was obvious that FGF22 significantly increased cell viability of 3T3 cells.
USAGE of the Active Fibroblast Growth Factor 22 (FGF22)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Fibroblast Growth Factor 22 (FGF22)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Fibroblast Growth Factor 22 (FGF22)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Catalog No.||Organism species: Mus musculus (Mouse)||Applications (RESEARCH USE ONLY!)|
|RPC909Mu01||Recombinant Fibroblast Growth Factor 22 (FGF22)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC909Mu01||Active Fibroblast Growth Factor 22 (FGF22)||Cell culture; Activity Assays.|
|PAC909Mu01||Polyclonal Antibody to Fibroblast Growth Factor 22 (FGF22)||WB; IHC; ICC; IP.|
|MEC909Mu||Mini Samples ELISA Kit for Fibroblast Growth Factor 22 (FGF22)||Enzyme-linked immunosorbent assay for Antigen Detection.|