Active Factor Related Apoptosis (FAS)

CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6

  • Active Factor Related Apoptosis (FAS) Packages (Simulation)
  • Active Factor Related Apoptosis (FAS) Packages (Simulation)
  • APA030Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Factor Related Apoptosis (FAS)

FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A binding ELISA assay was conducted to detect the association of FAS with FASL. Briefly, FASL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul FASL were then transferred to FAS-coated microtiter wells (1ug/ml, 100ul/well) and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FASL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and FASL was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 was approximately 0.012 ug/mL.

USAGE of the Active Factor Related Apoptosis (FAS)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Factor Related Apoptosis (FAS)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Factor Related Apoptosis (FAS)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA030Hu02 Recombinant Factor Related Apoptosis (FAS) Positive Control; Immunogen; SDS-PAGE; WB.
RPA030Hu03 Recombinant Factor Related Apoptosis (FAS) Positive Control; Immunogen; SDS-PAGE; WB.
RPA030Hu01 Recombinant Factor Related Apoptosis (FAS) Positive Control; Immunogen; SDS-PAGE; WB.
APA030Hu03 Active Factor Related Apoptosis (FAS) Cell culture; Activity Assays.
EPA030Hu61 Eukaryotic Factor Related Apoptosis (FAS) Positive Control; Immunogen; SDS-PAGE; WB.
APA030Hu61 Active Factor Related Apoptosis (FAS) Cell culture; Activity Assays.
APA030Hu01 Active Factor Related Apoptosis (FAS) Cell culture; Activity Assays.
PAA030Hu02 Polyclonal Antibody to Factor Related Apoptosis (FAS) WB; IHC
PAA030Hu03 Polyclonal Antibody to Factor Related Apoptosis (FAS) WB; IHC; ICC; IP.
PAA030Hu01 Polyclonal Antibody to Factor Related Apoptosis (FAS) WB; IHC; ICC; IP.
FAA030Hu01 Anti-Factor Related Apoptosis (FAS) Polyclonal Antibody Flow cytometry.
LAA030Hu71 Biotin-Linked Polyclonal Antibody to Factor Related Apoptosis (FAS) WB; IHC; ICC.
LAA030Hu81 FITC-Linked Polyclonal Antibody to Factor Related Apoptosis (FAS) WB; IHC; ICC; IF.
FAA030Hu41 PE-Linked Anti-Factor Related Apoptosis (FAS) Polyclonal Antibody Flow cytometry.
FAA030Hu51 APC-Linked Anti-Factor Related Apoptosis (FAS) Polyclonal Antibody Flow cytometry.
FAA030Hu81 FITC-Linked Anti-Factor Related Apoptosis (FAS) Polyclonal Antibody Flow cytometry.
MAA030Hu22 Monoclonal Antibody to Factor Related Apoptosis (FAS) WB; IHC; ICC; IP.
SEA030Hu ELISA Kit for Factor Related Apoptosis (FAS) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA030Hu CLIA Kit for Factor Related Apoptosis (FAS) Chemiluminescent immunoassay for Antigen Detection.
LMA030Hu Multiplex Assay Kit for Factor Related Apoptosis (FAS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA030Hu01 ELISA Kit DIY Materials for Factor Related Apoptosis (FAS) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK