Active Epidermal Growth Factor (EGF)
URG; Beta-Urogastrone; Pro-epidermal growth factor
- Product No.APA560Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point4.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 128
For more details, please contact local distributors!US$ 320
For more details, please contact local distributors! US$ 640
For more details, please contact local distributors! US$ 1920
For more details, please contact local distributors! US$ 4800
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant EGF, Human.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Epidermal Growth Factor (EGF)
Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .
Cell proliferation of 3T3 cells after incubation with EGF for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of EGF was shown in Figure 2. It was obvious that EGF significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 0.9848 to 2.958ng/mL.
USAGE of the Active Epidermal Growth Factor (EGF)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Epidermal Growth Factor (EGF)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Epidermal Growth Factor (EGF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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