Active Elastase 4 (ELA4)

CLCR; CTRC; Chymotrypsin C; Caldecrin

  • Active Elastase 4 (ELA4)Packages (Simulation)
  • Active Elastase 4 (ELA4)Packages (Simulation)
  • APB310Ra01.jpgSDS-PAGE
  • Active Elastase 4 (ELA4)Figure. Western Blot; Sample: Recombinant ELA4, Rat.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Elastase 4 (ELA4)

Elastase 4 (ELA4) is a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. It regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ELA4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ELA4 and recombinant rat uPAR. Briefly, ELA4 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ELA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ELA4 and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Elastase 4 (ELA4)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Elastase 4 (ELA4)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Elastase 4 (ELA4)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Related products

Catalog No.Organism species: Rattus norvegicus (Rat)Applications (RESEARCH USE ONLY!)
RPB310Ra01Recombinant Elastase 4 (ELA4)Positive Control; Immunogen; SDS-PAGE; WB.
APB310Ra01Active Elastase 4 (ELA4)Cell culture; Activity Assays.
PAB310Ra01Polyclonal Antibody to Elastase 4 (ELA4)WB; IHC; ICC; IP.
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