Active Diacylglycerol Kinase Epsilon (DGKe)
DAGK5; DGK; Diglyceride kinase epsilon
- Product No.APE848Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point7.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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ACTIVITY TEST of the Active Diacylglycerol Kinase Epsilon (DGKe)
DGKe (Diacylglycerol kinase epsilon) is an enzyme that mainly involved in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha) has been proven to be an interactor of DGKe through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human DGKe and recombinant human PDHA. Briefly, DGKe were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL DGKe were then transferred to PDHA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-DGKe pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DGKe and PDHA was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Diacylglycerol Kinase Epsilon (DGKe)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Diacylglycerol Kinase Epsilon (DGKe)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Diacylglycerol Kinase Epsilon (DGKe)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPE848Hu01 | Recombinant Diacylglycerol Kinase Epsilon (DGKe) | Positive Control; Immunogen; SDS-PAGE; WB. |
APE848Hu01 | Active Diacylglycerol Kinase Epsilon (DGKe) | Cell culture; Activity Assays. |
PAE848Hu01 | Polyclonal Antibody to Diacylglycerol Kinase Epsilon (DGKe) | WB; IHC; ICC; IP. |