Active Coagulation Factor II (F2)

FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2

  • Active Coagulation Factor II (F2)Packages (Simulation)
  • Active Coagulation Factor II (F2)Packages (Simulation)
  • Active Coagulation Factor II (F2)Gene sequencing
  • APA820Ra02.jpgSDS-PAGE
  • Active Coagulation Factor II (F2)Figure. Western Blot; Sample: Recombinant F2, Human.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Coagulation Factor II (F2)

Coagulation Factor II (F2) also commonly called pro-thrombin is a coagulation protein in the blood stream that has many effects in the coagulation cascade. It is a serine protease that converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Besides, Protein C Inhibitor (PCI) has been identified as an interactor of F2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat F2 and recombinant rat PCI. Briefly, F2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PCI-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-F2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of F2 and PCI was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Coagulation Factor II (F2)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Coagulation Factor II (F2)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Coagulation Factor II (F2)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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