Active Arylsulfatase B (ARSB)
ASB; G4S; N-acetylgalactosamine-4-sulfatase
- Product No.APG610Hu01
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 80%
- Isoelectric Point7.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 376
For more details, please contact local distributors!US$ 940
For more details, please contact local distributors!US$ 1880
For more details, please contact local distributors!US$ 5640
For more details, please contact local distributors!US$ 141000
For more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant ARSB, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Arylsulfatase B (ARSB)
Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Arylsulfatase B (ARSB)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Arylsulfatase B (ARSB)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Arylsulfatase B (ARSB)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|APG610Hu01||Active Arylsulfatase B (ARSB)||Cell culture; Activity Assays.|
|RPG610Hu01||Recombinant Arylsulfatase B (ARSB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAG610Hu01||Polyclonal Antibody to Arylsulfatase B (ARSB)||WB; IHC; ICC; IP.|
|MAG610Hu21||Monoclonal Antibody to Arylsulfatase B (ARSB)||WB; IHC; ICC; IP.|
|SEG610Hu||ELISA Kit for Arylsulfatase B (ARSB)||Enzyme-linked immunosorbent assay for Antigen Detection.|