ELISA Kit DIY Materials for Calreticulin (CRT)

CALR; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

  • ELISA Kit DIY Materials for Calreticulin (CRT) Packages (Simulation)
  • ELISA Kit DIY Materials for Calreticulin (CRT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the ELISA Kit DIY Materials for Calreticulin (CRT)

The Abs in the kit have high sensitivity and excellent specificity for detection of Calreticulin (CRT). No significant cross-reactivity or interference between Calreticulin (CRT) and analogues was observed.

USAGE of the ELISA Kit DIY Materials for Calreticulin (CRT)

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE of the ELISA Kit DIY Materials for Calreticulin (CRT)

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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